RP-HPLC method development for the simultaneous determination of timolol maleate and human serum albumin in albumin nanoparticles
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چکیده
منابع مشابه
Development and validation of RP-HPLC and RP-UPLC methods for quantification of erythropoietin formulated with human serum albumin
Rapid and sensitive reverse phase high performance liquid chromatography (RP-HPLC) and ultra performance liquid chromatography (UPLC) methods with UV detection for quantification of erythropoietin (EPO) in presence of human serum albumin (HSA) as a stabilizer in a pharmaceutical formulation of EPO have been developed and validated. Chromatography was performed with mobile phase containing 0.1% ...
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A simple HPLC with UV detection method is developed and validated for determination of Bovine Serum Albumin and Myoglobin proteins in a standard solution of the two protein as well as in a plasma spiked with theses two proteins. Separation was achieved on a reversed-phase C18, 5.0 μm, 150mm × 4.6 mm inner diameter column using a mobile phase consisting of solution A (900 mL of water, 100 mL Ace...
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Background and Aims: Some nanoparticles can be used in immunoassays to increase sensitivity. This study aimed to evaluate a novel nano-immunoassay based on bovine serum albumin nanoparticles (BSA NPs). Materials and methods: At first, the nanostructure was synthesized, and then applied as a tag in the nano-immunoassay. Then the concentration of β-subunit of human chorionic gonadotropin ...
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A rapid, sensitive and specific RP-HPLC method involving UV detection was developed [1-15] and validated [16-21] for determination and quantification of Amoxycillin and Potassium Clavulunate. Chromatography was carried out on a pre-packed Hypersil C18 (5μm, 250x4.6mm) column using filtered and degassed mixture of Phosphate buffer:Methanol (95:05) as mobile phase at a flow rate of 1.0ml/min and ...
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a simple, rapid and low-cost scanner spectroscopy method for the glucose determination by utilizing glucose oxidase and cdte/tga quantum dots as chromoionophore has been described. the detection was based on the combination of the glucose enzymatic reaction and the quenching effect of h2o2 on the cdte quantum dots (qds) photoluminescence.in this study glucose was determined by utilizing glucose...
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ژورنال
عنوان ژورنال: Journal of Pharmaceutical and Biomedical Analysis
سال: 2015
ISSN: 0731-7085
DOI: 10.1016/j.jpba.2015.03.031